Seminal plasma metabolomics profiles following long (4–7 days) and short (2 h) sexual abstinence periods

ObjectiveMetabolomic profiling of seminal plasma has been suggested as a possible approach for fast and non-invasive male infertility evaluation diagnosis. However, metabolomics profiles in normozoospermic men have not thoroughly investigated, the influence ejaculation-abstinence described.To provide interim reference values find associations between human length period men.Study designSemen samples collected after long (4–7 days) short abstinence (2 h) from 31 males were assessed routine quality parameters before was separated by centrifugation. Metabolomics then determined using untargeted Nuclear Magnetic Resonance Spectroscopy.ResultsIn total, 30 metabolites identified. Pyruvate showed higher concentration, while fructose, acetate, choline, methanol, N-acetylglucosamine, O-acetylcarnitine, uridine, sn-glycero-3-phosphocoline lower concentrations (vs. long). All absolute amounts (volume × concentration) following shorter abstinence. sperm concentration resulted pyruvate taurine per spermatozoa: 1.92 (1.12–3.87) vs. 1.29 (0.83–2.62) (P < 0.001) 0.58 (0.36–0.92) 0.43 (0.28–0.95) 0.05) ng/106 spermatozoa. Simultaneously, there percentage progressively motile spermatozoa abstinence.ConclusionThe generally periods may be related to time available secretion collection these accessory glands epididymides. The concomitant number second ejaculate increased spermatozoa, accompanied motility samples.The simultaneous increase percentages might indicate that two play more critical role motility, which should further investigated future studies.